Sanger sequencing, also known as the “chain termination method,” was developed by Frederick Sanger and his colleagues in 1977. This method is used for determining the sequence of nucleotide bases in a piece of DNA. Sanger sequencing with 99.99% base accuracy is considered the “gold standard” for validating DNA sequences, including those already sequenced through next-generation sequencing (NGS).

• chevron_rightSanger sequencing is recommended in order to rule out false positives (for low sequencing depth variants).
• chevron_rightPrenatal testing and Diagnosis of already diagnosed families.

Sanger sequencing remains widely used in the sequencing field due to its several prominent advantages.

• chevron_rightCost-efficiency for sequencing single genes and 99.99% accuracy, especially suitable for verification sequencing for site-directed mutagenesis or cloned inserts.
• chevron_rightSanger sequencing is a robust testing strategy able to determine whether a point mutation or small deletion/duplication is present.
• chevron_rightTargeting smaller genomic regions in a larger number of samples.
• chevron_rightSequencing of variable regions.
• chevron_rightValidating results from next-generation sequencing (NGS) studies.
• chevron_rightVerifying plasmid sequences, inserts, mutations.
• chevron_rightHLA typing.
• chevron_rightGenotyping of microsatellite markers