Sanger sequencing, also known as the “chain termination method,” was developed by Frederick Sanger and his colleagues in 1977. This method is used for determining the sequence of nucleotide bases in a piece of DNA. Sanger sequencing with 99.99% base accuracy is considered the “gold standard” for validating DNA sequences, including those already sequenced through next-generation sequencing (NGS).
Sanger sequencing remains widely used in the sequencing field due to its several prominent advantages.
Whole Blood: 2-4mL (4mL preferred) of whole blood in EDTA.
Sanger Sequencing Steps
The Sanger sequencing method consists of 6 steps: