Next Generation Sequencing | Sandor Speciality Diagnostics

Whole Genome Sequencing

Whole-genome sequencing (WGS) is the most relevant method for analyzing the complete genome. WGS has important advantages over other techniques, it identifies variations not only in the coding regions (exons) but also in non-coding regions (introns) that affect gene expression. This technique will help in identifying inherited disorders, characterizing the mutations that drive to different diseases. The ability to produce large volumes of data with today’s sequencers make whole-genome sequencing a powerful tool for genomics research.

Captures both large and small variants that might otherwise be missed.
Identifies potential causative variants for further follow-on studies of gene expression and regulation mechanisms.

Targeted resequencing – Analysis with known reference genome
Denovo Sequencing – Analysis of unknown genome

Transcriptome Sequencing

Whole Transcriptome or RNA sequencing enables quantification and characterization of RNA in a biological sample at a given moment in time.
Small RNAs are a type of non-coding RNA (ncRNA) molecules including miRNAs, SiRNAs and Piwi RNAs. They are often involved in regulating the translation of target

Small RNA Sequencing

Small RNAs are a type of non-coding RNA (ncRNA) molecules including miRNAs, SiRNAs and Piwi RNAs. They are often involved in regulating the translation of targets.
miRNA Seq : Sequencing of miRNA which usually range 16-35 bp, to know the following

Prediction of Novel miRNA (If genome sequence id known)
Target prediction Other Non-coding RNA Seq: Profiling of other non coding RNA ranging from 50- 250 bp including long non-coding RNA which are involved in chromatin modification and gene regulation.

Chip Sequencing

Chromatin Immunoprecipitation (ChIP) sequencing is a powerful method for identifying genome-wide DNA binding sites for transcription factors and histone proteins. ChIPseq combines chromatin immunoprecipitation with massively parallel DNA sequencing. We accept immunoprecipitated samples for library preparation and sequencing.

Captures DNA targets for transcription factors or histone proteins across the entire genome of any organism.
Defines transcription factor binding sites

Methylome Sequencing

DNA methylation is an epigenetic mechanism that occurs by the addition of a methyl (CH3) group to DNA to control gene expression. Cytosine methylation can significantly modify temporal & spatial gene expression and chromatin remodeling. Whole-genome bisulfite sequencing (WGBS) leverages the power of NGS to provide a comprehensive view of methylation patterns at single-base resolution across the genome.

Discover methylation patterns of all CpG and non CpG (CHH, and CHG) regions across the entire genome.

Metagenome Sequencing

Metagenomics is the study of organisms in a microbial community based on DNA analysis. Environmental metagenomics data can be used for agricultural microbiome analysis, ecological remediation, or other biological investigations.

16S rRNA Sequencing: identify and compare bacterial strains present within a given sample.

Amplicon Sequencing

Amplicon sequencing is a highly targeted approach for sequencing of PCR products (amplicons) that allows efficient variant identification and characterization. Offers efficiency for discovering, validating, and screening genetic variants.

Multiplexes thousand amplicons per reaction to achieve high coverage.
Delivers highly targeted resequencing even in difficult-to-sequence areas, such as GC-rich regions.
Allows flexibility for a wide range of experimental designs.
Reduces sequencing costs and turnaround time compared to broader approaches.

Genotyping by Sequencing(GBS)

Genotyping by Sequencing (GBS) is the cost effective technique to discover and score SNPs in order to perform genotyping studies, such as genome-wide association studies (GWAS). GBS uses restriction enzymes to reduce genome complexity and genotype multiple samples.

Provides a low cost per sample.
Reduces ascertainment bias compared to arrays.
Identifies variants other than SNPs, including small insertions, deletions, and microsatellites.
Enables comparative analyses across samples in the absence of a reference genome.
Informs genetic mapping, screening backcross lines, purity testing, constructing haplotype maps, association mapping, and genomic selection for plant studies.

NGS Applications

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